23227/23225/pierce BCA 试剂盒现货

ThermoFisher	23227	Pierce™ BCA Protein Assay Kit	500mL
ThermoFisher	23225	Pierce™ BCA Protein Assay Kit	1L



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描述

The Thermo Scientific Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) total protein concentration compared to a protein standard.

Features of the BCA Protein Assay Kit:

• Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader (562nm)
• Excellent uniformity—exhibits less protein-to-protein variation than dye-binding methods
• Compatible—unaffected by typical concentrations of most ionic and nonionic detergents
• Moderay fast—much easier and four times faster than the classical Lowry method
• High linearity—linear working range for BSA equals 20 to 2000 µg/mL
• Sensitive—detect down to 5 µg/mL with the enhanced protocol

Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Applications:
• Studying protein:protein interactions
• Measuring column fractions after affinity chromatography
• Estimating percent recovery of membrane proteins from cell extracts
• High-throughput screening of fusion proteins

How the BCA Protein Assay Detects Protein:
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid. The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.

In the second step of the color development reaction, bicinchoninic acid (BCA) reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water-soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The BCA reagent is approximay 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.

The reaction that leads to BCA color formation is strongly influenced by four amino acid residues (cysteine or cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike the Coomassie dye-binding methods, the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.

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蚂蚁淘常见平行进口产品有：
Thermofisher旗下Gibco培养系列、ABI细胞计数仪及PCR仪、Invitrogen分子生物学试剂及Qubit3.0、Ambion的RNA相关试剂、Molecular probes试剂、Pierce蛋白相关试剂盒、Nanodrop One/OneC；Parafilm封口膜；Viskase透析袋；Sigma-Aldrich试剂；AccuStandard 标准品；Millipore抗体；Glen Research 核酸合成试剂；Bio-Rad电泳仪及PCR仪；Nanocs的PEG聚合物；  Chromotek羊驼抗体；   Polysciences转染试剂；  Echelon-inc 脂类研究试剂盒；   R&D Systems抗体及Elisa试剂盒； Jackson二抗及封闭血清； Biotium 荧光染料；NEB内切酶及NGS相关产品；  Athens Research & Technology人类蛋白及抗血清；  Illumina二代测试试剂盒； Tocris 小分子试剂； TwistDx恒温扩增试剂盒（RPA）； Enzymatics二代测序酶；Novus抗体及Elisa试剂盒；AG scientific潮霉素B、G-418；Plantmedia植物培养系列及抗体；Agilent 2100生物分析仪；Biolog耗材；Biomatik的Elisa试剂盒；Covaris基因打断仪；CST抗体；Dharmacon的siRNA产品。